Kanamycin Aesculin Azide Agar Base 500g

Kanamycin Aesculin Azide Agar Base 500g is used for the isolation and enumeration of Enterococci, especially Enterococcus species from different specimens like food, water, etc. This agar composition contains kanamycin to suppress the growth of most Gram-negative bacilli and sodium azide as an additional selective agent, inhibiting the growth of other Gram-positive bacilli, except enterococci. Aesculin in the medium causes enterococci to be differentiated according to their content of aesculin, and brown colouring of the medium indicates their presence. Kana Agar Base is commonly used in general microbiological testing in environmental and food industries, as well as in clinical laboratories.

Key Features : Kanamycin Aesculin Azide Agar Base

Enterococci Selective: Kanamycin and sodium azide are available as selective agents for the growth of Gram-positive organisms, while inhibiting the growth of Gram-negative bacteria. This allows for the development of enterococci from mixed cultures (e.g., environmental, water, food samples).
Differential Medium for Aesculin Hydrolysis: Aesculin is included in the medium as a source of differential substrates to differentiate enterococci from other Gram-positive cocci. Enterococci produce the enzyme aesculinase, which can hydrolyse aesculin, forming a black-brown complex in the medium. This is a unique feature of enterococci and aids in their differentiation from other organisms.
Robust Performance: This medium is being type-optimised for use in a variety of applications which demand reliable and reproducible results. The presence of selective agents (kanamycin and sodium azide) and the aesculin hydrolysis characteristic allows only enterococci to grow and to be differentiated from non-enterococcal bacteria.
Versatile: Kanamycin Aesculin Azide Agar Base is employed in a variety of microbiological applications, including water testing, food microbiology, and clinical testing, making it a versatile and indispensable addition to your laboratory testing supplies.
High-Quality Formulation: The agar base is manufactured with only high-quality ingredients to provide consistent and reproducible results in microbial isolation and differentiation. The medium is free from non-bacterial impurities and has been formulated to meet industry standards for bacteriological applications.

Specifications :Kanamycin Aesculin Azide Agar Base

Cat No: Kanamycin Aesculin Azide Agar Base 500g
Amount: 500g (Dehydrated powder)
Storage: Store in a cool, dry place, away from direct sunlight and moisture. When ready, the medium can be stored at 4°C until it is used.
Application: This agar medium is for the isolation of enterococci from clinical, food, and environmental specimens.
pH: Normally about 7.2 ± 0.2
Preparation: Prepare the medium in distilled water by heating it to a boil to ensure complete dissolution of the medium. Autoclave at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and add supplements or egg yolk if necessary.

Applications

Water Testing: Kanamycin Aesculin Azide Agar Base is recommended for testing water for the presence and enumeration of enterococci, particularly in environmental and recreational water samples. Enterococci are a significant indicator of faecal contamination in water; therefore, this medium is also essential for testing water.
Food Microbiology: This agar is also used to isolate enterococci from food samples, where these organisms may appear due to contamination during processing or improper handling. Enterococci are also relevant in the context of food safety tests and spoilage investigations.
Clinical Microbiology: Kanamycin-Aesculin-Azide Agar Base is recommended as a primary culture medium in clinical laboratories to produce enterococci from specimens submitted by patients, such as blood, urine, and wound cultures. Enterococci are responsible for a wide range of infections, including urinary tract infections, bacteremia, and endocarditis, where identification is essential for patient diagnosis and treatment.
Environmental Monitoring: Utilised in environmental investigations to monitor and assess soil, wastewater, and other environmental samples for microbial contamination, aiding in the identification and control of hazards.

Why choose Kanamycin Aesculin Azide Agar Base 500g from Bright?

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