Trypsin 2000

Product Description

Trypsin 2000 (Ex Pancreas) (Code: 06310) is a high-purity proteolytic enzyme derived from porcine pancreas, specially refined for biological, biochemical, and cell culture applications. It is a serine protease that catalyzes the hydrolysis of peptide bonds, specifically at the carboxyl side of lysine and arginine residues, resulting in the controlled digestion of proteins.

With an enzyme activity of approximately 2000 USP units/mg, Trypsin 2000 is ideal for tissue dissociation, cell detachment, protein sequencing, and peptide mapping. It is available in both powder and solution forms, ensuring flexibility for various laboratory procedures. Its high activity, purity, and reproducibility make it a preferred reagent for researchers working in molecular biology, enzymology, and bioprocessing.

Key Features

• Product Code: 06310
• Chemical Name: Trypsin (Ex Pancreas)
• Source: Porcine Pancreas
• Enzyme Commission Number (EC): 3.4.21.4
• Activity: ~2000 USP units/mg protein
• Appearance: White to pale yellow crystalline or amorphous powder
• Molecular Weight: ~23,800 Da
• Optimum pH: 7.5–8.5
• Optimum Temperature: 37°C
• Solubility: Freely soluble in water or buffer (phosphate/saline)
• Storage: 2–8°C (refrigerated); avoid repeated freeze–thaw cycles
• Stabilizer: May contain calcium salts for enhanced enzyme stability

Applications

Laboratory & Research

• Cell Culture: Used for detaching adherent cells during subculturing and cell passaging.
• Protein Chemistry: Hydrolyzes proteins for peptide analysis and amino acid sequencing.
• Enzymology: Serves as a model protease in biochemical mechanism studies.
• Tissue Dissociation: Facilitates isolation of primary cells from animal tissues.
• Biotechnology: Employed in protein modification and purification processes.

Industrial & Educational Uses

• Pharmaceutical & Biotech Industries: Applied in enzyme formulations, diagnostic reagents, and therapeutic research.
• Academic Laboratories: Used for teaching enzymology, biochemistry, and molecular biology experiments.
• Food & Bioprocessing: Supports specialized protein hydrolysis and bioconversion studies.

Safety & Handling

• Hazard: May cause irritation to skin, eyes, or respiratory tract. Enzymes can induce allergic reactions upon repeated exposure.
• Precautions: Use gloves, goggles, and protective clothing. Handle in a fume hood or well-ventilated area to avoid inhalation of enzyme dust.
• Storage: Store in a tightly sealed container at 2–8°C, away from moisture and light.
• Disposal: Dispose of enzyme solutions and residues in accordance with biological waste regulations.

Price in Bangladesh

Available in 10 g, 25 g, and 100 g packs, or custom bulk quantities upon request.
Enzyme activity certification provided with every batch.
Competitive pricing with prompt nationwide delivery.

Why Choose Trypsin 2000 (Ex Pancreas) 06310 from M/S Bright Scientific Co.?

• High-activity enzyme (~2000 USP units/mg) for consistent, reliable performance.
• Derived from high-quality pancreatic source material.
• Suitable for a wide range of cell culture and biochemical applications.
• Available in research and bulk-grade packaging.
• Trusted nationwide by universities, life science institutes, and biotechnology companies.

Contact Information

M/S Bright Scientific Co.
📞 Phone: 01711285976