Product Description
Phenol Chloroform Isoamyl Alcohol (25:24:1) pH 8.0 for Molecular Biology
Appearance:
- Clarity: Clear
- Colour: Pale Yellow
- Form: Liquid
pH: ~ 8.0
DNase, RNase, Protease: Not detected
Stabilizer: 0.1% 8-Hydroxyquinoline
Code: 69031
Brand: SRL
Origin: India
Formula: NA
CAS: 136112-00-0
Pack Size: 500ml
Chemical Product and Company Identification
Product Name: Phenol:Chloroform:Isoamyl Alcohol (25:24:1) pH 8.0 for Molecular Biology
Product Code: 69031
CAS No: 136112-00-0
Use: Laboratory Chemicals
Company Address:
608, B Wing, Satellite Gazebo, Andheri Ghatkopar Link Road,
Andheri (E), Mumbai – 400 099, India
Company Name: Sisco Research Laboratories Pvt. Ltd.
Composition/Information on Ingredients
| CAS# | Chemical Name | % | EINECS# |
|---|---|---|---|
| 136112-00-0 | Phenol Chloroform Isoamyl Alcohol | 100 | 203-632-7 |
No components need to be disclosed according to the applicable regulations.
Hazards Identification
Risk advice to man and the environment:
- Toxic if swallowed.
- Very toxic in contact with skin.
- Irritating to eyes, respiratory system, and skin.
First Aid Measures
Eyes: Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician.
Skin: Wash off with soap and plenty of water. Take victim immediately to hospital. Consult a physician.
Ingestion: Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician.
Inhalation: If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult a physician.
General advice: Consult a physician. Show this safety data sheet to the doctor in attendance.
Fire Fighting Measures
Extinguishing Media:
- Suitable: Use water spray, alcohol-resistant foam, dry chemical, or carbon dioxide.
Special Protective Equipment for Firefighters:
- Wear self-contained breathing apparatus for fire fighting if necessary.
How Phenol Chloroform Isoamyl Alcohol Works in Molecular Biology
1. Cell Lysis
After cells are broken open, the Phenol Chloroform Isoamyl Alcohol (PCI) mixture is added to the sample.
2. Protein Denaturation
Phenol in Phenol Chloroform Isoamyl Alcohol denatures proteins, causing them to unfold and become insoluble.
3. Layer Separation
The mixture separates into two layers:
- Upper Aqueous Layer: Contains polar nucleic acids.
- Lower Organic Layer: Contains denatured proteins and lipids.
Chloroform helps to create a clear separation between these layers.
4. Foam Prevention
Isoamyl alcohol (Isopentyl alcohol) is added to prevent the formation of stable foam during mixing.
5. Nucleic Acid Recovery
The purified nucleic acid (DNA or RNA) is in the aqueous phase and can be collected for downstream applications, such as reverse transcription, PCR, or DNA fingerprinting.
Safety Precautions for Phenol Chloroform Isoamyl Alcohol (PCI)
Toxicity: PCI is a highly toxic mixture.
Corrosivity: It causes severe skin burns and eye damage.
Handling: Always wear appropriate personal protective equipment (PPE), including gloves, eye protection, and a face shield.
Fume Hood: Perform all work with PCI in a well-ventilated fume hood.
Waste Disposal: Dispose of waste according to local guidelines for hazardous materials.
Uses of Phenol Chloroform Isoamyl Alcohol
RNA Isolation
Isolating RNA from various tissues for gene expression studies.
DNA Extraction
Extracting high-quality DNA for genetic analysis applications.
Variations for DNA and RNA Extraction Using Phenol Chloroform Isoamyl Alcohol
For DNA Extraction
At pH 7.2–8.0, DNA remains in the aqueous phase, while RNA, lipids, and proteins move to the organic phase and interphase.
For RNA Extraction
An acidic pH (around 4.3–4.7) is used. Under these conditions, DNA is forced into the organic layer, allowing for the specific isolation of RNA in the aqueous phase.
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Phenol Chloroform Isoamyl Alcohol Price in Bangladesh
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